Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic aspen under semi-natural conditions.
Identifieur interne : 000031 ( Main/Exploration ); précédent : 000030; suivant : 000032Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic aspen under semi-natural conditions.
Auteurs : Elena O. Vidyagina ; Natalia M. Subbotina ; Vladimir A. Belyi ; Vadim G. Lebedev ; Konstantin V. Krutovsky [Allemagne, États-Unis] ; Konstantin A. ShestibratovSource :
- BMC plant biology [ 1471-2229 ] ; 2020.
Abstract
BACKGROUND
Recombinant carbohydrases genes are used to produce transgenic woody plants with improved phenotypic traits. However, cultivation of such plants in open field is challenging due to a number of problems. Therefore, additional research is needed to alleviate them.
RESULTS
Results of successful cultivation of the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions are reported in this paper for the first time. Change of carbohydrate composition of wood was observed in transgenic aspens carrying the sp-Xeg gene. The transformed transgenic line Xeg-2-1b demonstrated accelerated growth and increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt. The accelerated growth was observed also in the transgenic line Xeg-1-1c. Thicker cell-wall and longer xylem fiber were also observed in both these transgenic lines. Undescribed earlier considerable reduction in the wood decomposition rate of the transgenic aspen stems was also revealed for the transformed transgenic lines. The decomposition rate was approximately twice as lower for the transgenic line Xeg-2-3b in comparison with the control untransformed line Pt.
CONCLUSION
A direct dependence of the phenotypic and biochemical traits on the expression of the recombinant gene sp-Xeg was demonstrated. The higher was the level of the sp-Xeg gene expression, the more pronounced were changes in the phenotypic and biochemical traits. All lines showed phenotypic changes in the leave traits. Our results showed that the plants carrying the recombinant sp-Xeg gene do not demonstrate a decrease in growth parameters in semi-natural conditions. In some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness, and a decrease in the rate of decomposition of wood were observed.
DOI: 10.1186/s12870-020-02469-2
PubMed: 32493269
PubMed Central: PMC7268456
Affiliations:
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Subbotina</name><affiliation><nlm:affiliation>Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Prospect Nauki 6, Pushchino, Russian Federation, 142290.</nlm:affiliation><wicri:noCountry code="subField">142290</wicri:noCountry></affiliation></author><author><name sortKey="Belyi, Vladimir A" sort="Belyi, Vladimir A" uniqKey="Belyi V" first="Vladimir A" last="Belyi">Vladimir A. Belyi</name><affiliation><nlm:affiliation>Institute of Сhemistry, Komi Science Centre, Urals Branch of the Russian Academy of Sciences, Republic of Komi, Pervomaiskaya Str. 48, Syktyvkar, Russian Federation, 167000.</nlm:affiliation><wicri:noCountry code="subField">167000</wicri:noCountry></affiliation></author><author><name sortKey="Lebedev, Vadim G" sort="Lebedev, Vadim G" uniqKey="Lebedev V" first="Vadim G" last="Lebedev">Vadim G. 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Shestibratov</name><affiliation><nlm:affiliation>Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Prospect Nauki 6, Pushchino, Russian Federation, 142290.</nlm:affiliation><wicri:noCountry code="subField">142290</wicri:noCountry></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32493269</idno><idno type="pmid">32493269</idno><idno type="doi">10.1186/s12870-020-02469-2</idno><idno type="pmc">PMC7268456</idno><idno type="wicri:Area/Main/Corpus">000269</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000269</idno><idno type="wicri:Area/Main/Curation">000269</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000269</idno><idno type="wicri:Area/Main/Exploration">000269</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic aspen under semi-natural conditions.</title><author><name sortKey="Vidyagina, Elena O" sort="Vidyagina, Elena O" uniqKey="Vidyagina E" first="Elena O" last="Vidyagina">Elena O. Vidyagina</name><affiliation><nlm:affiliation>Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Prospect Nauki 6, Pushchino, Russian Federation, 142290.</nlm:affiliation><wicri:noCountry code="subField">142290</wicri:noCountry></affiliation></author><author><name sortKey="Subbotina, Natalia M" sort="Subbotina, Natalia M" uniqKey="Subbotina N" first="Natalia M" last="Subbotina">Natalia M. Subbotina</name><affiliation><nlm:affiliation>Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Prospect Nauki 6, Pushchino, Russian Federation, 142290.</nlm:affiliation><wicri:noCountry code="subField">142290</wicri:noCountry></affiliation></author><author><name sortKey="Belyi, Vladimir A" sort="Belyi, Vladimir A" uniqKey="Belyi V" first="Vladimir A" last="Belyi">Vladimir A. Belyi</name><affiliation><nlm:affiliation>Institute of Сhemistry, Komi Science Centre, Urals Branch of the Russian Academy of Sciences, Republic of Komi, Pervomaiskaya Str. 48, Syktyvkar, Russian Federation, 167000.</nlm:affiliation><wicri:noCountry code="subField">167000</wicri:noCountry></affiliation></author><author><name sortKey="Lebedev, Vadim G" sort="Lebedev, Vadim G" uniqKey="Lebedev V" first="Vadim G" last="Lebedev">Vadim G. Lebedev</name><affiliation><nlm:affiliation>Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Prospect Nauki 6, Pushchino, Russian Federation, 142290.</nlm:affiliation><wicri:noCountry code="subField">142290</wicri:noCountry></affiliation></author><author><name sortKey="Krutovsky, Konstantin V" sort="Krutovsky, Konstantin V" uniqKey="Krutovsky K" first="Konstantin V" last="Krutovsky">Konstantin V. Krutovsky</name><affiliation wicri:level="3"><nlm:affiliation>Department of Forest Genetics and Forest Tree Breeding, George-August University of Göttingen, Büsgenweg 2, 37077, Göttingen, Germany. konstantin.krutovsky@forst.uni-goettingen.de.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Forest Genetics and Forest Tree Breeding, George-August University of Göttingen, Büsgenweg 2, 37077, Göttingen</wicri:regionArea><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Göttingen</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Center for Integrated Breeding Research, George-August University of Göttingen, Albrecht-Thaer-Weg 3, 37077, Göttingen, Germany. konstantin.krutovsky@forst.uni-goettingen.de.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Center for Integrated Breeding Research, George-August University of Göttingen, Albrecht-Thaer-Weg 3, 37077, Göttingen</wicri:regionArea><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Göttingen</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Population Genetics, N. I. Vavilov Institute of General Genetics, Russian Academy of Sciences, Gubkina Str. 3, Moscow, Russian Federation, 119991. konstantin.krutovsky@forst.uni-goettingen.de.</nlm:affiliation><country wicri:rule="url">Allemagne</country></affiliation><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Forest Genomics, Genomic Research and Education Center, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Akademgorodok 50a/2, Krasnoyarsk, Russian Federation, 660036. konstantin.krutovsky@forst.uni-goettingen.de.</nlm:affiliation><country wicri:rule="url">Allemagne</country></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Ecosystem Sciences and Management, Texas A&M University, College Station, TX, 77843-2138, USA. konstantin.krutovsky@forst.uni-goettingen.de.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Ecosystem Sciences and Management, Texas A&M University, College Station, TX, 77843-2138</wicri:regionArea><wicri:noRegion>77843-2138</wicri:noRegion></affiliation></author><author><name sortKey="Shestibratov, Konstantin A" sort="Shestibratov, Konstantin A" uniqKey="Shestibratov K" first="Konstantin A" last="Shestibratov">Konstantin A. Shestibratov</name><affiliation><nlm:affiliation>Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Prospect Nauki 6, Pushchino, Russian Federation, 142290.</nlm:affiliation><wicri:noCountry code="subField">142290</wicri:noCountry></affiliation></author></analytic><series><title level="j">BMC plant biology</title><idno type="eISSN">1471-2229</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>Recombinant carbohydrases genes are used to produce transgenic woody plants with improved phenotypic traits. However, cultivation of such plants in open field is challenging due to a number of problems. Therefore, additional research is needed to alleviate them.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>Results of successful cultivation of the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions are reported in this paper for the first time. Change of carbohydrate composition of wood was observed in transgenic aspens carrying the sp-Xeg gene. The transformed transgenic line Xeg-2-1b demonstrated accelerated growth and increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt. The accelerated growth was observed also in the transgenic line Xeg-1-1c. Thicker cell-wall and longer xylem fiber were also observed in both these transgenic lines. Undescribed earlier considerable reduction in the wood decomposition rate of the transgenic aspen stems was also revealed for the transformed transgenic lines. The decomposition rate was approximately twice as lower for the transgenic line Xeg-2-3b in comparison with the control untransformed line Pt.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSION</b></p><p>A direct dependence of the phenotypic and biochemical traits on the expression of the recombinant gene sp-Xeg was demonstrated. The higher was the level of the sp-Xeg gene expression, the more pronounced were changes in the phenotypic and biochemical traits. All lines showed phenotypic changes in the leave traits. Our results showed that the plants carrying the recombinant sp-Xeg gene do not demonstrate a decrease in growth parameters in semi-natural conditions. In some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness, and a decrease in the rate of decomposition of wood were observed.</p></div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32493269</PMID><DateRevised><Year>2020</Year><Month>06</Month><Day>09</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1471-2229</ISSN><JournalIssue CitedMedium="Internet"><Volume>20</Volume><Issue>1</Issue><PubDate><Year>2020</Year><Month>Jun</Month><Day>03</Day></PubDate></JournalIssue><Title>BMC plant biology</Title><ISOAbbreviation>BMC Plant Biol</ISOAbbreviation></Journal><ArticleTitle>Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic aspen under semi-natural conditions.</ArticleTitle><Pagination><MedlinePgn>251</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s12870-020-02469-2</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Recombinant carbohydrases genes are used to produce transgenic woody plants with improved phenotypic traits. However, cultivation of such plants in open field is challenging due to a number of problems. Therefore, additional research is needed to alleviate them.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Results of successful cultivation of the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions are reported in this paper for the first time. Change of carbohydrate composition of wood was observed in transgenic aspens carrying the sp-Xeg gene. The transformed transgenic line Xeg-2-1b demonstrated accelerated growth and increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt. The accelerated growth was observed also in the transgenic line Xeg-1-1c. Thicker cell-wall and longer xylem fiber were also observed in both these transgenic lines. Undescribed earlier considerable reduction in the wood decomposition rate of the transgenic aspen stems was also revealed for the transformed transgenic lines. The decomposition rate was approximately twice as lower for the transgenic line Xeg-2-3b in comparison with the control untransformed line Pt.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">A direct dependence of the phenotypic and biochemical traits on the expression of the recombinant gene sp-Xeg was demonstrated. The higher was the level of the sp-Xeg gene expression, the more pronounced were changes in the phenotypic and biochemical traits. All lines showed phenotypic changes in the leave traits. Our results showed that the plants carrying the recombinant sp-Xeg gene do not demonstrate a decrease in growth parameters in semi-natural conditions. In some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness, and a decrease in the rate of decomposition of wood were observed.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Vidyagina</LastName><ForeName>Elena O</ForeName><Initials>EO</Initials><AffiliationInfo><Affiliation>Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Prospect Nauki 6, Pushchino, Russian Federation, 142290.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Subbotina</LastName><ForeName>Natalia M</ForeName><Initials>NM</Initials><AffiliationInfo><Affiliation>Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Prospect Nauki 6, Pushchino, Russian Federation, 142290.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Belyi</LastName><ForeName>Vladimir A</ForeName><Initials>VA</Initials><AffiliationInfo><Affiliation>Institute of Сhemistry, Komi Science Centre, Urals Branch of the Russian Academy of Sciences, Republic of Komi, Pervomaiskaya Str. 48, Syktyvkar, Russian Federation, 167000.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lebedev</LastName><ForeName>Vadim G</ForeName><Initials>VG</Initials><AffiliationInfo><Affiliation>Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Prospect Nauki 6, Pushchino, Russian Federation, 142290.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Krutovsky</LastName><ForeName>Konstantin V</ForeName><Initials>KV</Initials><Identifier Source="ORCID">https://orcid.org/0000-0002-8819-7084</Identifier><AffiliationInfo><Affiliation>Department of Forest Genetics and Forest Tree Breeding, George-August University of Göttingen, Büsgenweg 2, 37077, Göttingen, Germany. konstantin.krutovsky@forst.uni-goettingen.de.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Center for Integrated Breeding Research, George-August University of Göttingen, Albrecht-Thaer-Weg 3, 37077, Göttingen, Germany. konstantin.krutovsky@forst.uni-goettingen.de.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Laboratory of Population Genetics, N. I. Vavilov Institute of General Genetics, Russian Academy of Sciences, Gubkina Str. 3, Moscow, Russian Federation, 119991. konstantin.krutovsky@forst.uni-goettingen.de.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Laboratory of Forest Genomics, Genomic Research and Education Center, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Akademgorodok 50a/2, Krasnoyarsk, Russian Federation, 660036. konstantin.krutovsky@forst.uni-goettingen.de.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Ecosystem Sciences and Management, Texas A&M University, College Station, TX, 77843-2138, USA. konstantin.krutovsky@forst.uni-goettingen.de.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shestibratov</LastName><ForeName>Konstantin A</ForeName><Initials>KA</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-8819-7084</Identifier><AffiliationInfo><Affiliation>Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Prospect Nauki 6, Pushchino, Russian Federation, 142290.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>0101-2019-0037</GrantID><Agency>Ministry of Science and High Education of the Russian Federation</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>06</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>BMC Plant Biol</MedlineTA><NlmUniqueID>100967807</NlmUniqueID><ISSNLinking>1471-2229</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Aspen</Keyword><Keyword MajorTopicYN="N">Gene expression level</Keyword><Keyword MajorTopicYN="N">Penicillium canescens</Keyword><Keyword MajorTopicYN="N">Populus tremula</Keyword><Keyword MajorTopicYN="N">Transgenic</Keyword><Keyword MajorTopicYN="N">Xyloglucanase</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>07</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>05</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>6</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>6</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>6</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32493269</ArticleId><ArticleId IdType="doi">10.1186/s12870-020-02469-2</ArticleId><ArticleId IdType="pii">10.1186/s12870-020-02469-2</ArticleId><ArticleId IdType="pmc">PMC7268456</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Plant Cell. 2007 Jun;19(6):1947-63</Citation><ArticleIdList><ArticleId IdType="pubmed">17557806</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 1999 Nov;210(1):19-26</Citation><ArticleIdList><ArticleId IdType="pubmed">10592028</ArticleId></ArticleIdList></Reference><Reference><Citation>Stain Technol. 1974 Jan;49(1):9-14</Citation><ArticleIdList><ArticleId IdType="pubmed">4131626</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Commun. 2016 Jun 28;7:11989</Citation><ArticleIdList><ArticleId IdType="pubmed">27349324</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Biofuels. 2015 Mar 12;8:41</Citation><ArticleIdList><ArticleId IdType="pubmed">25802552</ArticleId></ArticleIdList></Reference><Reference><Citation>Anat Rec. 1964 Oct;150:129-39</Citation><ArticleIdList><ArticleId IdType="pubmed">14223588</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Biotechnol J. 2017 Oct;15(10):1309-1321</Citation><ArticleIdList><ArticleId IdType="pubmed">28258966</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Biotechnol J. 2016 Jan;14(1):299-312</Citation><ArticleIdList><ArticleId IdType="pubmed">25923308</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Plant Biol. 2011 Aug 25;11:119</Citation><ArticleIdList><ArticleId IdType="pubmed">21867507</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2003 Mar;33(6):1099-106</Citation><ArticleIdList><ArticleId IdType="pubmed">12631333</ArticleId></ArticleIdList></Reference><Reference><Citation>Ann Bot. 2008 Nov;102(5):659-65</Citation><ArticleIdList><ArticleId IdType="pubmed">18757879</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2002 Dec;14(12):3073-88</Citation><ArticleIdList><ArticleId IdType="pubmed">12468728</ArticleId></ArticleIdList></Reference><Reference><Citation>Tree Physiol. 2009 May;29(5):725-36</Citation><ArticleIdList><ArticleId IdType="pubmed">19324699</ArticleId></ArticleIdList></Reference><Reference><Citation>Ecology. 2010 Dec;91(12):3686-97</Citation><ArticleIdList><ArticleId IdType="pubmed">21302839</ArticleId></ArticleIdList></Reference><Reference><Citation>J Plant Res. 2006 Jan;119(1):37-42</Citation><ArticleIdList><ArticleId IdType="pubmed">16284709</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2014 Jan 14;111(2):845-50</Citation><ArticleIdList><ArticleId IdType="pubmed">24379366</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Plant Sci. 2013 Dec 13;4:507</Citation><ArticleIdList><ArticleId IdType="pubmed">24379822</ArticleId></ArticleIdList></Reference><Reference><Citation>Tree Physiol. 2014 Nov;34(11):1289-300</Citation><ArticleIdList><ArticleId IdType="pubmed">24728296</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2007 Aug;51(3):485-99</Citation><ArticleIdList><ArticleId IdType="pubmed">17587235</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 1970 Aug 15;227(5259):680-5</Citation><ArticleIdList><ArticleId IdType="pubmed">5432063</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2014 Apr;164(4):1789-99</Citation><ArticleIdList><ArticleId IdType="pubmed">24572173</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Rep. 2016 Aug 24;6:31066</Citation><ArticleIdList><ArticleId IdType="pubmed">27553882</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant. 2009 Sep;2(5):893-903</Citation><ArticleIdList><ArticleId IdType="pubmed">19825666</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant. 2011 Jan;4(1):17-24</Citation><ArticleIdList><ArticleId IdType="pubmed">20943810</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):9055-60</Citation><ArticleIdList><ArticleId IdType="pubmed">12084943</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Plant Biol. 2008 Jul 22;8:82</Citation><ArticleIdList><ArticleId IdType="pubmed">18647399</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4939-44</Citation><ArticleIdList><ArticleId IdType="pubmed">12668766</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2008;177(3):589-607</Citation><ArticleIdList><ArticleId IdType="pubmed">18211474</ArticleId></ArticleIdList></Reference><Reference><Citation>FEBS Lett. 2004 Apr 23;564(1-2):183-7</Citation><ArticleIdList><ArticleId IdType="pubmed">15094064</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1999 Aug 3;96(16):9433-7</Citation><ArticleIdList><ArticleId IdType="pubmed">10430960</ArticleId></ArticleIdList></Reference><Reference><Citation>Ecol Lett. 2009 Jan;12(1):45-56</Citation><ArticleIdList><ArticleId IdType="pubmed">19016827</ArticleId></ArticleIdList></Reference><Reference><Citation>J Vis Exp. 2010 Mar 12;(37):</Citation><ArticleIdList><ArticleId IdType="pubmed">20228730</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2010 Jul;153(3):1261-79</Citation><ArticleIdList><ArticleId IdType="pubmed">20460583</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2008 Aug 8;283(32):21853-63</Citation><ArticleIdList><ArticleId IdType="pubmed">18511421</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1947 Feb 21;105(2721):214</Citation><ArticleIdList><ArticleId IdType="pubmed">17737883</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li><li>États-Unis</li></country><region><li>Basse-Saxe</li></region><settlement><li>Göttingen</li></settlement></list><tree><noCountry><name sortKey="Belyi, Vladimir A" sort="Belyi, Vladimir A" uniqKey="Belyi V" first="Vladimir A" last="Belyi">Vladimir A. 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Krutovsky</name><name sortKey="Krutovsky, Konstantin V" sort="Krutovsky, Konstantin V" uniqKey="Krutovsky K" first="Konstantin V" last="Krutovsky">Konstantin V. Krutovsky</name><name sortKey="Krutovsky, Konstantin V" sort="Krutovsky, Konstantin V" uniqKey="Krutovsky K" first="Konstantin V" last="Krutovsky">Konstantin V. Krutovsky</name></country><country name="États-Unis"><noRegion><name sortKey="Krutovsky, Konstantin V" sort="Krutovsky, Konstantin V" uniqKey="Krutovsky K" first="Konstantin V" last="Krutovsky">Konstantin V. Krutovsky</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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